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skeletal polya mrna provided by the pcr select kit clontech  (TaKaRa)


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    TaKaRa skeletal polya mrna provided by the pcr select kit clontech
    Skeletal Polya Mrna Provided By The Pcr Select Kit Clontech, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+poly+a+selected+rna/Mouse+Skeletal+Muscle+Poly+A%2B+RNA/10__17660_slash_actahortic__2001__543__18-76-7-15
    Average 94 stars, based on 10 article reviews
    skeletal polya mrna provided by the pcr select kit clontech - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Northern Blot:

    Article Title: Cloning and developmental expression of the murine homolog of the acute leukemia proto-oncogene AF4.
    Article Snippet: The green ̄uorescence was detected with a ZEISS ̄uorescent microscope using 450 ± 490 nm excitation ®lter, 490 ± 585 nm readout ®lter and photographed. .. Mouse poly-A selected RNA from heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis was prepared and a Northern blot (CLONETECH, Palo Alto, CA) was hybridized sequentially with 32P-labeled murine Af4 cDNA pz5b, the 303 base pair Af4 subclone used as a template for riboprobe generation, a human Af4 cDNA probe, the house keeping gene GAPDH and a cross-hybridizing human b-actin probe. .. The results were visualized by autoradiography with exposures ranging from 16 ± 60 h. In situ hybridization In situ hybridization (Sasaki and Hogan, 1993; Sitzman et al., 1995) was carried out according to the mafacturer's protocol using digoxigenin labelled uridine triphosphate as substrate by in vitro transcription (Boehringer Mannheim, Indianapolis.).



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    skeletal polya mrna provided by the pcr select kit clontech  (TaKaRa)
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    Cloning of human 60-kDa SS-A/Ro–associated protein pp75. (a) Schematic representation of cDNAs pINP1 derived from yeast 2-hybrid cloning, 5′ RACE, WI38 cDNA library screen, and clone KIAA0857. The shaded boxes represent the open reading frame (ORF). (b) Nucleotide and deduced amino acid sequence of pp75/KIAA0857. Nucleotide sequence of 3,017 bases for the WI38 cDNA was submitted to GenBank under accession number AF153085. The nucleotide sequences corresponding to the ORF between sequence submitted under AF153085 and clone KIAA0857 are identical; 4 single base substitutions and insertion/deletion are observed in the 3′ untranslated regions. The methionine start site (Kozak’s sequence CCGCCATGG) and <t>poly-A</t> signal AATAAA are underlined. The GC-rich 5′-untranslated region is highlighted by a thick underline of 5 or more consecutive GC residues. The protein has relatively high percentage of serine residues (13.94%; boldface and italics). A leucine zipper motif is double-underlined, and the 4 leucine residues are circled. RT-PCR primers and antisense RNA probe for the analysis of <t>mRNA</t> expression in different cells and tissues are indicated by arrows in both panels. The BamHI site used to generate antisense RNA probe is boxed in b.
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    Cloning of human 60-kDa SS-A/Ro–associated protein pp75. (a) Schematic representation of cDNAs pINP1 derived from yeast 2-hybrid cloning, 5′ RACE, WI38 cDNA library screen, and clone KIAA0857. The shaded boxes represent the open reading frame (ORF). (b) Nucleotide and deduced amino acid sequence of pp75/KIAA0857. Nucleotide sequence of 3,017 bases for the WI38 cDNA was submitted to GenBank under accession number AF153085. The nucleotide sequences corresponding to the ORF between sequence submitted under AF153085 and clone KIAA0857 are identical; 4 single base substitutions and insertion/deletion are observed in the 3′ untranslated regions. The methionine start site (Kozak’s sequence CCGCCATGG) and <t>poly-A</t> signal AATAAA are underlined. The GC-rich 5′-untranslated region is highlighted by a thick underline of 5 or more consecutive GC residues. The protein has relatively high percentage of serine residues (13.94%; boldface and italics). A leucine zipper motif is double-underlined, and the 4 leucine residues are circled. RT-PCR primers and antisense RNA probe for the analysis of <t>mRNA</t> expression in different cells and tissues are indicated by arrows in both panels. The BamHI site used to generate antisense RNA probe is boxed in b.
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    Image Search Results


    Cloning of human 60-kDa SS-A/Ro–associated protein pp75. (a) Schematic representation of cDNAs pINP1 derived from yeast 2-hybrid cloning, 5′ RACE, WI38 cDNA library screen, and clone KIAA0857. The shaded boxes represent the open reading frame (ORF). (b) Nucleotide and deduced amino acid sequence of pp75/KIAA0857. Nucleotide sequence of 3,017 bases for the WI38 cDNA was submitted to GenBank under accession number AF153085. The nucleotide sequences corresponding to the ORF between sequence submitted under AF153085 and clone KIAA0857 are identical; 4 single base substitutions and insertion/deletion are observed in the 3′ untranslated regions. The methionine start site (Kozak’s sequence CCGCCATGG) and poly-A signal AATAAA are underlined. The GC-rich 5′-untranslated region is highlighted by a thick underline of 5 or more consecutive GC residues. The protein has relatively high percentage of serine residues (13.94%; boldface and italics). A leucine zipper motif is double-underlined, and the 4 leucine residues are circled. RT-PCR primers and antisense RNA probe for the analysis of mRNA expression in different cells and tissues are indicated by arrows in both panels. The BamHI site used to generate antisense RNA probe is boxed in b.

    Journal:

    Article Title: Defining a novel 75-kDa phosphoprotein associated with SS-A/Ro and identification of distinct human autoantibodies

    doi:

    Figure Lengend Snippet: Cloning of human 60-kDa SS-A/Ro–associated protein pp75. (a) Schematic representation of cDNAs pINP1 derived from yeast 2-hybrid cloning, 5′ RACE, WI38 cDNA library screen, and clone KIAA0857. The shaded boxes represent the open reading frame (ORF). (b) Nucleotide and deduced amino acid sequence of pp75/KIAA0857. Nucleotide sequence of 3,017 bases for the WI38 cDNA was submitted to GenBank under accession number AF153085. The nucleotide sequences corresponding to the ORF between sequence submitted under AF153085 and clone KIAA0857 are identical; 4 single base substitutions and insertion/deletion are observed in the 3′ untranslated regions. The methionine start site (Kozak’s sequence CCGCCATGG) and poly-A signal AATAAA are underlined. The GC-rich 5′-untranslated region is highlighted by a thick underline of 5 or more consecutive GC residues. The protein has relatively high percentage of serine residues (13.94%; boldface and italics). A leucine zipper motif is double-underlined, and the 4 leucine residues are circled. RT-PCR primers and antisense RNA probe for the analysis of mRNA expression in different cells and tissues are indicated by arrows in both panels. The BamHI site used to generate antisense RNA probe is boxed in b.

    Article Snippet: A multiple tissue Northern blot with 2 μg poly-A selected mRNA on each lane was purchased from CLONTECH.

    Techniques: Clone Assay, Derivative Assay, cDNA Library Assay, Sequencing, Reverse Transcription Polymerase Chain Reaction, Expressing